Monitoring nitrogen utilization is crucial in agricultural practices, emphasizing the interrelationship between soil health, nutrient management, and human health. The study was conducted to evaluate the impact of N fertilizer on the nutritional characteristics of diverse S. bicolor varieties, namely Alföldi 1, ES Föehn (Lidea Seeds) with a red pericarp, ES Albanus, Albita, and Farmsugro 180 (all white varieties), the study was conducted in sorghum-producing areas where the crop is non-native. Specifically, the study investigated two soil types: loam clay and sandy soil. Furthermore, the respective varieties were grown under N (27% N CAN) fertilizer conditions, involving 60 kg/ha-1 and 120 kg/ha-1 of the treatment rates applied at each experiment site. We measured the specific element concentration in each sample using the Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) technology. Certainly, the results demonstrated that the different S. bicolor varieties had unique nutritional characteristics attributed to several factors such as soil type, variety, and treatment, which showed a significance value of (P  0.05) when considering the year factor, which supported the data analysis's reliability and accuracy. In summary, to enhance the nutritional value of sorghum grain and supply nutrient-rich food choices for individuals, consider factors such as fertilizer response, nutrient uptake by grain, element mineral accumulation, and advisory variety. Additional research could enhance the nutritional properties of sorghum to provide the required dietary stuff, such as grain processing, which can render sorghum a proper addition to a healthy and balanced human diet.